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GlycoSens™ Rat Prostaglandin E3 (PGE3) Assay Kit

CAT#: GLJF-0926-HX1548

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The GlycoSens™ Rat Prostaglandin E3 (PGE3) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of rat prostaglandin E3. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Other Series
Target
PGE3
Kit Components
Pre-coated Microplate; Biotinylated Tracer; HRP-Streptavidin Conjugate; Standard Solution; Assay Diluent Buffer; Wash Buffer; TMB Chromogenic Substrate; Stop Solution
Sample Types
Serum, plasma, tissue homogenates, cell culture supernatants, urine, biological fluids
Sample Volume
5 μL - 20 μL
Detection Principle
Target rat prostaglandin E3 (PGE3) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target PGE3.
Detection Time
Within 2 hours
Detection Wavelength
450 nm
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Species
Rat
Full Name
Prostaglandin E3
Alternate Names
RatE3; PGE3; Prostaglandin E3
Application Notes
Background
As a pivotal biomolecule, rat prostaglandin E3 (PGE3) plays a central role in complex physiological networks. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. In addition, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.
Application
Tailored for accurate quantification of rat prostaglandin E3 (PGE3) across cell biology, organelle bioenergetics, and intracellular signaling cascades.
Key Features & Advantages
High Specificity; Stable Reaction; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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