GlycoSens™ Transhydrogenase-1 (TH-1) Assay Kit

The GlycoSens™ Transhydrogenase-1 (TH-1) Assay Kit provides an advanced, quantitative micro-volume spectrophotometric method for determining TH-1 in biological specimens. Featuring superior kinetic linearity and high throughput compatibility, it facilitates accurate biochemical profiling.

Oxidative Phosphorylation Series

TH-1

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

TH-1 catalyzes hydride transfer from NADH to NADP⁺. Synthetic analog 3-acetylpyridine adenine dinucleotide phosphate (APADP⁺) replaces NADP⁺ and is reduced to APADPH, which absorbs specifically at 375 nm. Activity is calculated by monitoring absorbance accumulation rate at 375 nm.

Within 1 hour

375 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Transhydrogenase-1

Transhydrogenase-1; TH-1; Transhydrogenase-1 Standard

As a pivotal biomolecule, transhydrogenase-1 (TH-1) plays a central role in complex physiological networks. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. In addition, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Specifically engineered for evaluating transhydrogenase-1 (TH-1) dynamics in cell biology, pyridine nucleotide regulation, and mitochondrial membrane bioenergetics research.

High Specificity; Rapid Reaction Protocol; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.