GlycoSens™ Transhydrogenase-1 (TH-1) Assay Kit, Spectrophotometry

The GlycoSens™ Transhydrogenase-1 (TH-1) Assay Kit provides an advanced, quantitative spectrophotometric method for determining TH-1 in biological specimens. Featuring superior kinetic linearity and high throughput compatibility, it facilitates accurate biochemical profiling.

Oxidative Phosphorylation Series

TH-1

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

TH-1 catalyzes hydride transfer from NADH to NADP⁺. Synthetic analog 3-acetylpyridine adenine dinucleotide phosphate (APADP⁺) replaces NADP⁺ and is reduced to APADPH, which absorbs specifically at 375 nm. Activity is calculated by monitoring absorbance accumulation rate at 375 nm.

Within 1 hour

375 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Transhydrogenase-1

Transhydrogenase-1; TH-1; Transhydrogenase-1 Standard

Known for its specific biological characteristics, transhydrogenase-1 (TH-1) provides deep insights into cellular dynamics. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. As a result, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Widely applicable for tracking transhydrogenase-1 (TH-1) fluctuations across cellular respiration studies, electron transport dynamics, and metabolic flux profiling.

Stable Signal Output; Broad Sample Compatibility; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.