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Quantitative Human 5HTR7 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX378

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Specifications
Product Description
The quantitative human 5HTR7 (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 7 (5HTR7) levels. 5HTR7 is a G protein-coupled receptor, which binds to serotonin. 5HTR7 plays a role in regulating circadian rhythms. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.098 ng/mL, which can accurately detect low concentrations of 5HTR7 in the sample.
Target
5HTR7
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.098 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
5-Hydroxytryptamine receptor 7
Alternate Names
5HTR7; 5-Hydroxytryptamine receptor 7; 5-HT7; Serotonin receptor 7
Uniprot No.
N-Glycosylation Site
5, 66
Application Notes
Application
The quantitative human 5HTR7 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated 5HTR7 levels. It is useful for studying the role of 5HTR7 in regulating sleep-wake cycles.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-105%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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