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Quantitative Human ABCB5 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX623)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ABCB5 (glycosylated) sandwich ELISA kit is designed to detect human ATP binding cassette transporter B5 (ABCB5) levels. ABCB5 is a transmembrane protein that functions as an ATP-dependent drug efflux pump, which belongs to the ABC transporter superfamily. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.087 ng/mL, which can accurately detect low concentrations of ABCB5 in the sample.
Target ABCB5
N-Glycosylation Site 17, 85, 91, 371, 390, 423, 789, 819, 910, 1104, 1188
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.087 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name ATP binding cassette transporter B5
Alternate Names ABCB5; ATP binding cassette transporter B5; P-Glycoprotein ABCB5
Uniprot No. Q2M3G0
Application The quantitative human ABCB5 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ABCB5 levels in biological samples. The kit is applicable in studies of drug resistance, cancer biology, and ABC transporter functions. It is useful in fields such as oncology and pharmacology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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