Quantitative Human ABCB5 (Glycosylated) Sandwich ELISA Kit

The quantitative human ABCB5 (glycosylated) sandwich ELISA kit is designed to detect human ATP binding cassette transporter B5 (ABCB5) levels. ABCB5 is a transmembrane protein that functions as an ATP-dependent drug efflux pump, which belongs to the ABC transporter superfamily. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.087 ng/mL, which can accurately detect low concentrations of ABCB5 in the sample.

ABCB5

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.087 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

ATP binding cassette transporter B5

ABCB5; ATP binding cassette transporter B5; P-Glycoprotein ABCB5

17, 85, 91, 371, 390, 423, 789, 819, 910, 1104, 1188

The quantitative human ABCB5 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ABCB5 levels in biological samples. The kit is applicable in studies of drug resistance, cancer biology, and ABC transporter functions. It is useful in fields such as oncology and pharmacology.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.