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Quantitative Human Alk-Smase (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX648)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human Alk-Smase (glycosylated) sandwich ELISA kit is designed to detect human alkaline sphingomyelinase (Alk-Smase) levels. Alk-Smase catalyzes the hydrolysis of sphingomyelin, a type of lipid found in cell membranes, to produce ceramide and phosphocholine. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.112 ng/mL, which can accurately detect low concentrations of Alk-Smase in the sample.
Target Alk-Smase
N-Glycosylation Site 100, 121, 146, 168, 267
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.112 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Alkaline sphingomyelinase
Alternate Names Alk-Smase; Alkaline sphingomyelinase; ENPP7; NPP-7; Sphingomyelin phosphodiesterase
Uniprot No. Q6UWV6
Application The quantitative human Alk-Smase (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated alk-smase levels in biological samples. The kit is applicable in studies of lipid metabolism, cell signaling, and ceramide production. It is useful in fields such as biochemistry and cell biology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-110%;
Plasma sample: n=4, 85-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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