Quantitative Human Alk-Smase (Glycosylated) Sandwich ELISA Kit

The quantitative human Alk-Smase (glycosylated) sandwich ELISA kit is designed to detect human alkaline sphingomyelinase (Alk-Smase) levels. Alk-Smase catalyzes the hydrolysis of sphingomyelin, a type of lipid found in cell membranes, to produce ceramide and phosphocholine. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.112 ng/mL, which can accurately detect low concentrations of Alk-Smase in the sample.

Alk-Smase

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.112 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Alkaline sphingomyelinase

Alk-Smase; Alkaline sphingomyelinase; ENPP7; NPP-7; Sphingomyelin phosphodiesterase

100, 121, 146, 168, 267

The quantitative human Alk-Smase (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated alk-smase levels in biological samples. The kit is applicable in studies of lipid metabolism, cell signaling, and ceramide production. It is useful in fields such as biochemistry and cell biology.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-110%;
Plasma sample: n=4, 85-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.