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Quantitative Human CACNα1B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX508

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Specifications
Product Description
The quantitative human CACNα1B (glycosylated) sandwich ELISA kit is designed to detect human calcium channel, N-type, α1B subunit (CACNα1B) levels. CACNα1B forms a critical part of a protein complex that mediates calcium ion influx into cells. These channels are essential for various cellular processes, including neurotransmitter release, muscle contraction, and hormone secretion. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.134 ng/mL, which can accurately detect low concentrations of CACNα1B in the sample.
Target
CACNα1B
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.134 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Calcium channel, N-type, α1B subunit
Alternate Names
CACNα1B; Calcium channel, N-type, α1B subunit; CACNN; CACNL1A5; Brain calcium channel III
Uniprot No.
N-Glycosylation Site
256, 1563, 1675
Application Notes
Application
The quantitative human CACNα1B (glycosylated) sandwich ELISA kit is a valuable tool for neuroscience research, allowing scientists to study the expression and regulation of N-type calcium channels. Researchers use it to investigate the role of CACNα1B in neuronal functions and neurological disorders. The kit has applications in studies of pain, epilepsy, and other conditions involving altered calcium signaling in the nervous system.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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