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Quantitative Human CCL14 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX616

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Specifications
Product Description
The quantitative human CCL14 (glycosylated) sandwich ELISA kit is designed to detect human chemokine C-C-motif ligand 14 (CCL14) levels. CCL14 is a chemokine that attracts immune cells, particularly monocytes and macrophages, to sites of inflammation. This protein plays a role in the migration and activation of leukocytes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 6.835 pg/mL, which can accurately detect low concentrations of CCL14 in the sample.
Target
CCL14
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
6.835 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 pg/mL-800 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Chemokine C-C-motif ligand 14
Alternate Names
CCL14; Chemokine C-C-motif ligand 14; SCYA14; MCIF
Uniprot No.
O-Glycosylation Site
26
Application Notes
Application
The quantitative human CCL14 (glycosylated) sandwich ELISA kit is designed to determine the CCL14 concentration in biological samples. The kit finds application in research related to immunology, inflammation, and leukocyte trafficking. It is valuable in studies investigating the role of chemokines in immune responses.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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