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Quantitative Human CD3d (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX168)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CD3d (glycosylated) sandwich ELISA kit is designed to detect human cluster of differentiation 3d (CD3d) levels. CD3d is a subunit of the CD3 complex, which is essential for T cell receptor (TCR) signaling and T cell activation. It plays a crucial role in transmitting signals upon antigen recognition to initiate T cell responses. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.135 ng/mL, which can accurately detect low concentrations of CD3d in the sample.
Target CD3d
N-Glycosylation Site 38, 74
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.135 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Cluster of differentiation 3d
Alternate Names CD3d; Cluster of differentiation 3d; CD3-DELTA; T3D; T-cell receptor T3 delta chain
Uniprot No. P04234
Application The quantitative human CD3d (glycosylated) sandwich ELISA kit is valuable in research investigating T cell activation, immune responses, and disorders involving T cell dysfunction, such as autoimmune diseases and cancers. Researchers employ this ELISA to quantify CD3d levels, providing insights into T cell activity and potential therapeutic targets.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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