Quantitative Human COL1α1 (Glycosylated) Sandwich ELISA Kit

The quantitative human COL1α1 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 1 α1 (COL1α1) levels. COL1α1 is a major component of the extracellular matrix, forming the fibrillar collagen that provides structural support to various tissues, including skin, bone, and tendons. Its proper function is essential for tissue integrity and repair. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.281 ng/mL, which can accurately detect low concentrations of COL1α1 in the sample.

COL1α1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Serum, plasma

100 μL

0.281 ng/mL

Quantitative sandwich ELISA

2.55 ng/mL-50 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Collagen type 1 α1

COL1α1; Collagen type 1 α1; COL1-A1; Collagen α1(I) chain

1365

265, 1108

The quantitative human COL1α1 (glycosylated) sandwich ELISA kit is utilized in research investigating fibrosis, wound healing, and bone metabolism, as well as in studies related to connective tissue disorders. Researchers employ this ELISA to quantify COL1α1 levels, providing insights into its role in tissue remodeling and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.