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| Size | Qty | Add To Basket |
|---|---|---|
| 1×48 T | ||
| 1×96 T |
| Product Description | The quantitative human COL4α3 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 4α3 (COL4α3) levels. COL4α3 is a protein that forms a crucial component of type IV collagen. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.825 ng/mL, which can accurately detect low concentrations of COL4α3 in the sample. |
| Target | COL4α3 |
| N-Glycosylation Site | 253 |
| Sample Types | Tissue homogenates, cell lysates |
| Sample Volume | 100 μL |
| Sensitivity | 0.825 ng/mL |
| Detection Principle | Quantitative sandwich ELISA |
| Detection Range | 2.5 ng/mL-80 ng/mL |
| Detection Time | 1 h-5 h |
| Detection Wavelength | 450 nm |
| Storage | Store at 2-8°C for long term storage. |
| Species | Human |
| Full Name | Collagen type 4α3 |
| Alternate Names | COL4α3; Collagen type 4α3; COL4-A3; Collagen α3(IV) chain |
| Uniprot No. | Q01955 |
| Application | The quantitative human COL4α3 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and extracellular matrix biology, particularly in studies of basement membranes and collagen. Researchers use it to measure COL4α3 levels in tissue samples. The kit has applications in studies of kidney disease, and other conditions involving basement membrane abnormalities. |
| Kit Components | Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution |
| Precision | Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%; |
| Recovery | Serum sample: n=5, 90-105%; Plasma sample: n=4, 95-110%; |
| Standard Curve | ![]() The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested. |