Quantitative Human COL4α3 (Glycosylated) Sandwich ELISA Kit

The quantitative human COL4α3 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 4α3 (COL4α3) levels. COL4α3 is a protein that forms a crucial component of type IV collagen. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.825 ng/mL, which can accurately detect low concentrations of COL4α3 in the sample.

COL4α3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.825 ng/mL

Quantitative sandwich ELISA

2.5 ng/mL-80 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Collagen type 4α3

COL4α3; Collagen type 4α3; COL4-A3; Collagen α3(IV) chain

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The quantitative human COL4α3 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and extracellular matrix biology, particularly in studies of basement membranes and collagen. Researchers use it to measure COL4α3 levels in tissue samples. The kit has applications in studies of kidney disease, and other conditions involving basement membrane abnormalities.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 95-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.