Quantitative Human COL5α2 (Glycosylated) Sandwich ELISA Kit

The quantitative human COL5α2 (glycosylated) sandwich ELISA kit is designed to detect human collagen type V α2 (COL5α2) levels. COL5α2 is a fibrillar collagen that plays a critical role in regulating the assembly of collagen fibrils and providing structural support to various tissues. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.152 ng/mL, which can accurately detect low concentrations of COL5α2 in the sample.

COL5α2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.152 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Collagen type V α2

COL5α2; Collagen type V α2; COL5-A2; Collagen α2(V) chain

1262, 1400

The quantitative human COL5α2 (glycosylated) sandwich ELISA kit is valuable in research investigating connective tissue disorders, fibrosis, and wound healing, where COL5α2 levels are relevant. Researchers employ this ELISA to quantify COL5α2 levels, providing insights into its role in tissue remodeling and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.