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Quantitative Human COL5α2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX181)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL5α2 (glycosylated) sandwich ELISA kit is designed to detect human collagen type V α2 (COL5α2) levels. COL5α2 is a fibrillar collagen that plays a critical role in regulating the assembly of collagen fibrils and providing structural support to various tissues. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.152 ng/mL, which can accurately detect low concentrations of COL5α2 in the sample.
Target COL5α2
N-Glycosylation Site 1262, 1400
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.152 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type V α2
Alternate Names COL5α2; Collagen type V α2; COL5-A2; Collagen α2(V) chain
Uniprot No. P05997
Application The quantitative human COL5α2 (glycosylated) sandwich ELISA kit is valuable in research investigating connective tissue disorders, fibrosis, and wound healing, where COL5α2 levels are relevant. Researchers employ this ELISA to quantify COL5α2 levels, providing insights into its role in tissue remodeling and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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