Quantitative Human CTBS (Glycosylated) Sandwich ELISA Kit

The quantitative human CTBS (glycosylated) sandwich ELISA kit is designed to detect human di-N-acetyl chitobiase (CTBS) levels. CTBS is an enzyme involved in the biosynthesis of tetrahydrobiopterin (BH4). BH4 is an essential cofactor for several enzymes, including phenylalanine hydroxylase, tyrosine hydroxylase, and nitric oxide synthase. These enzymes play critical roles in amino acid metabolism, neurotransmitter synthesis, and nitric oxide production. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.085 ng/mL, which can accurately detect low concentrations of CTBS in the sample.

CTBS

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.085 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Di-N-acetyl chitobiase

CTBS; Di-N-acetyl chitobiase; CTB

193, 228, 262, 299

The quantitative human CTBS (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and metabolism, particularly in studies of BH4 metabolism and related disorders. Researchers use it to measure CTBS levels in biological samples. The kit has applications in studies of phenylketonuria, neurotransmitter deficiencies, and nitric oxide-related pathologies.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.