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Quantitative Human CTBS (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX512)

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1×48 T
1×96 T

Product Description The quantitative human CTBS (glycosylated) sandwich ELISA kit is designed to detect human di-N-acetyl chitobiase (CTBS) levels. CTBS is an enzyme involved in the biosynthesis of tetrahydrobiopterin (BH4). BH4 is an essential cofactor for several enzymes, including phenylalanine hydroxylase, tyrosine hydroxylase, and nitric oxide synthase. These enzymes play critical roles in amino acid metabolism, neurotransmitter synthesis, and nitric oxide production. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.085 ng/mL, which can accurately detect low concentrations of CTBS in the sample.
Target CTBS
N-Glycosylation Site 193, 228, 262, 299
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.085 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Di-N-acetyl chitobiase
Alternate Names CTBS; Di-N-acetyl chitobiase; CTB
Uniprot No. Q01459
Application The quantitative human CTBS (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and metabolism, particularly in studies of BH4 metabolism and related disorders. Researchers use it to measure CTBS levels in biological samples. The kit has applications in studies of phenylketonuria, neurotransmitter deficiencies, and nitric oxide-related pathologies.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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