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Quantitative Human EFNA5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX462

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Specifications
Product Description
The quantitative human EFNA5 (glycosylated) sandwich ELISA kit is designed to detect human ephrin A5 (EFNA5) levels. EFNA5 is another member of the ephrin-A protein family, which interacts with Eph receptors to mediate cell signaling. EFNA5 is involved in various developmental processes, including axon guidance. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.092 ng/mL, which can accurately detect low concentrations of EFNA5 in the sample.
Target
EFNA5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.092 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Ephrin A5
Alternate Names
EFNA5; Ephrin A5; AF1; EFL5; EPLG7; EPH-related receptor tyrosine kinase ligand 7
Uniprot No.
N-Glycosylation Site
37
Application Notes
Application
The quantitative human EFNA5 (glycosylated) sandwich ELISA kit is designed to measure glycosylated EFNA5 levels in samples. This measurement is valuable in research related to cell signaling. The kit is applied in studies of neural development, which helps in understanding the role of EFNA5.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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