Quantitative Human FFAR1 (Glycosylated) Sandwich ELISA Kit

The quantitative human FFAR1 (glycosylated) sandwich ELISA kit is designed to detect human free fatty acid receptor 1 (FFAR1) levels. FFAR1, also known as G protein-coupled receptor 40 (GPR40), is a G protein-coupled receptor that is activated by medium- and long-chain fatty acids, playing a role in glucose-stimulated insulin secretion and metabolic regulation. It is involved in nutrient sensing and energy homeostasis. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.932 ng/mL, which can accurately detect low concentrations of FFAR1 in the sample.

FFAR1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.932 ng/mL

Quantitative sandwich ELISA

5 ng/mL-100 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Free fatty acid receptor 1

FFAR1; Free fatty acid receptor 1; GPR40; FFA1R; G protein-coupled receptor 40

155

The quantitative human FFAR1 (glycosylated) sandwich ELISA kit is valuable for researchers investigating type 2 diabetes, obesity, and other metabolic disorders, where FFAR1 signaling is implicated. By quantifying FFAR1, researchers can gain insights into its role in disease pathogenesis and explore potential therapeutic targets related to glucose homeostasis and insulin secretion.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.