Quantitative Human HHIP (Glycosylated) Sandwich ELISA Kit

The quantitative human HHIP (glycosylated) sandwich ELISA kit is designed to detect human hedgehog interacting protein (HHIP) levels. HHIP is a protein that interacts with Hedgehog signaling molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.145 ng/mL, which can accurately detect low concentrations of HHIP in the sample.

HHIP

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.145 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Hedgehog interacting protein

HHIP; Hedgehog interacting protein; HIP

99, 416, 447, 459

The quantitative human HHIP (glycosylated) sandwich ELISA kit is designed to quantitatively measure glycosylated HHIP in samples. This quantification is important for research on Hedgehog signaling and embryonic development. The kit also has applications in investigations of cancer.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.