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Quantitative Human HHIP (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX750)

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1×48 T
1×96 T

Product Description The quantitative human HHIP (glycosylated) sandwich ELISA kit is designed to detect human hedgehog interacting protein (HHIP) levels. HHIP is a protein that interacts with Hedgehog signaling molecules. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.145 ng/mL, which can accurately detect low concentrations of HHIP in the sample.
Target HHIP
N-Glycosylation Site 99, 416, 447, 459
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.145 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Hedgehog interacting protein
Alternate Names HHIP; Hedgehog interacting protein; HIP
Uniprot No. Q96QV1
Application The quantitative human HHIP (glycosylated) sandwich ELISA kit is designed to quantitatively measure glycosylated HHIP in samples. This quantification is important for research on Hedgehog signaling and embryonic development. The kit also has applications in investigations of cancer.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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