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Quantitative Human IgM (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX143)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human IgM (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin M (IgM) levels. IgM is the first antibody produced during an immune response, playing a crucial role in early defense against pathogens. It is a pentameric antibody that effectively activates the complement system. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 15.137 ng/mL, which can accurately detect low concentrations of IgM in the sample.
Target IgM
N-Glycosylation Site 46, 209, 272, 279, 440
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 15.137 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 50 ng/mL-2000 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Immunoglobulin M
Alternate Names IgM; Immunoglobulin M; IGHM; Immunoglobulin heavy constant Mu
Uniprot No. P01871
Application The quantitative human IgM (glycosylated) sandwich ELISA kit is utilized in research and clinical settings to assess early immune responses, diagnose acute infections, and evaluate certain autoimmune diseases. Researchers and clinicians employ this ELISA to quantify IgM levels, providing valuable information about immune status and disease progression.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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