Quantitative Human IgM (Glycosylated) Sandwich ELISA Kit

The quantitative human IgM (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin M (IgM) levels. IgM is the first antibody produced during an immune response, playing a crucial role in early defense against pathogens. It is a pentameric antibody that effectively activates the complement system. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 15.137 ng/mL, which can accurately detect low concentrations of IgM in the sample.

IgM

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

15.137 ng/mL

Quantitative sandwich ELISA

50 ng/mL-2000 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Immunoglobulin M

IgM; Immunoglobulin M; IGHM; Immunoglobulin heavy constant Mu

46, 209, 272, 279, 440

The quantitative human IgM (glycosylated) sandwich ELISA kit is utilized in research and clinical settings to assess early immune responses, diagnose acute infections, and evaluate certain autoimmune diseases. Researchers and clinicians employ this ELISA to quantify IgM levels, providing valuable information about immune status and disease progression.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.