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Quantitative Human IL3Rα (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX338

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Specifications
Product Description
The quantitative human IL3Rα (glycosylated) sandwich ELISA kit is designed to detect human interleukin 3 receptor α (IL3Rα) levels. IL3Rα is a transmembrane protein, which binds to interleukin-3 (IL-3). IL3Rα plays a role in the proliferation of hematopoietic cells. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.145 ng/mL, which can accurately detect low concentrations of IL3Rα in the sample.
Target
IL3Rα
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.145 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Interleukin 3 receptor α
Alternate Names
IL3Rα; Interleukin 3 receptor α; CD123; IL3R-A; IL3R; IL3RAY
Uniprot No.
N-Glycosylation Site
46, 64, 80, 109, 212, 218
Application Notes
Application
The quantitative human IL3Rα (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated IL3Rα levels. It is useful for studying the role of IL3Rα in the growth of blood cells.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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