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Quantitative Human LIPH (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX711

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Specifications
Product Description
The quantitative human LIPH (glycosylated) sandwich ELISA kit is designed to detect human lipase H (LIPH) levels. LIPH is an enzyme, which belongs to the phospholipase A2 family. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.715 ng/mL, which can accurately detect low concentrations of LIPH in the sample.
Target
LIPH
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.715 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2.27 ng/mL-93 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Lipase H
Alternate Names
LIPH; Lipase H; LPDLR; PLA1B; LPD lipase-related protein; Phospholipase A1 member B
Uniprot No.
N-Glycosylation Site
50, 66, 357
Application Notes
Application
The quantitative human LIPH (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LIPH in samples. This measurement is valuable for research on lipid signaling and phospholipid metabolism. The kit also has potential applications in investigations of skin biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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