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Quantitative Human MAN2B1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX23)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human MAN2B1 (glycosylated) sandwich ELISA kit is designed to detect human mannosidase α class 2B member 1 (MAN2B1) levels. MAN2B1 is a lysosomal alpha-mannosidase involved in the degradation of N-linked oligosaccharides, playing a crucial role in glycoprotein processing and cellular homeostasis. It is essential for the proper functioning of the lysosomal degradation pathway. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 15.251 pg/mL, which can accurately detect low concentrations of MAN2B1 in the sample.
Target MAN2B1
N-Glycosylation Site 133, 310, 367, 497, 645, 651, 692, 766, 832, 930, 989
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 15.251 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 50 pg/mL-2000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Mannosidase α class 2B member 1
Alternate Names MAN2B1; Mannosidase α class 2B member 1; MANB; LAMAN; Lysosomal acid alpha-mannosidase; Mannosidase αB
Uniprot No. O00754
Application The quantitative human MAN2B1 (glycosylated) sandwich ELISA kit is valuable for researchers investigating lysosomal storage disorders, such as alpha-mannosidosis, where MAN2B1 deficiency leads to accumulation of mannose-rich oligosaccharides. By quantifying MAN2B1 levels, researchers can gain insights into disease pathogenesis, monitor disease progression, and evaluate potential therapeutic interventions related to lysosomal enzyme replacement or enhancement.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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