Quantitative Human MAN2B1 (Glycosylated) Sandwich ELISA Kit

The quantitative human MAN2B1 (glycosylated) sandwich ELISA kit is designed to detect human mannosidase α class 2B member 1 (MAN2B1) levels. MAN2B1 is a lysosomal alpha-mannosidase involved in the degradation of N-linked oligosaccharides, playing a crucial role in glycoprotein processing and cellular homeostasis. It is essential for the proper functioning of the lysosomal degradation pathway. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 15.251 pg/mL, which can accurately detect low concentrations of MAN2B1 in the sample.

MAN2B1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

15.251 pg/mL

Quantitative sandwich ELISA

50 pg/mL-2000 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Mannosidase α class 2B member 1

MAN2B1; Mannosidase α class 2B member 1; MANB; LAMAN; Lysosomal acid alpha-mannosidase; Mannosidase αB

133, 310, 367, 497, 645, 651, 692, 766, 832, 930, 989

The quantitative human MAN2B1 (glycosylated) sandwich ELISA kit is valuable for researchers investigating lysosomal storage disorders, such as alpha-mannosidosis, where MAN2B1 deficiency leads to accumulation of mannose-rich oligosaccharides. By quantifying MAN2B1 levels, researchers can gain insights into disease pathogenesis, monitor disease progression, and evaluate potential therapeutic interventions related to lysosomal enzyme replacement or enhancement.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.