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Quantitative Human NET (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX318

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Specifications
Product Description
The quantitative human NET (glycosylated) sandwich ELISA kit is designed to detect human norepinephrine transporter (NET) levels. NET is located in the nerve cell membrane, which is responsible for the reuptake of norepinephrine. This process removes norepinephrine from the synaptic cleft. NET plays a critical role in regulating neurotransmission. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.127 ng/mL, which can accurately detect low concentrations of NET in the sample.
Target
NET
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.127 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Norepinephrine transporter
Alternate Names
NET; Norepinephrine transporter; SLC6A2; NET1; Solute carrier family 6 member 2
Uniprot No.
N-Glycosylation Site
184, 192, 198
Application Notes
Application
The quantitative human NET (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated NET levels. It is useful for studying the role of NET in neurotransmission.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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