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Quantitative Human PCSK5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX719

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Specifications
Product Description
The quantitative human PCSK5 (glycosylated) sandwich ELISA kit is designed to detect human proprotein convertase subtilisin-kexin type 5 (PCSK5) levels. PCSK5 is an enzyme that belongs to the proprotein convertase family. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.084 ng/mL, which can accurately detect low concentrations of PCSK5 in the sample.
Target
PCSK5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.084 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Proprotein convertase subtilisin-kexin type 5
Alternate Names
PCSK5; Proprotein convertase subtilisin-kexin type 5; PC5; Proprotein convertase 5
Uniprot No.
N-Glycosylation Site
255, 381, 665, 752, 802, 852, 1014, 1191, 1290, 1497, 1685, 1707
Application Notes
Application
The quantitative human PCSK5 (glycosylated) sandwich ELISA kit provides a method for quantifying glycosylated PCSK5 in biological samples. This measurement is valuable for research on protein processing and enzyme functions. The kit also has potential applications in investigations of disease processes involving protein activation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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