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Quantitative Human WNT5A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1228

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human WNT5A (glycosylated) sandwich ELISA kit is designed to detect human wingless-type MMTV integration site family, member 5A (WNT5A) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.044 ng/mL, which can accurately detect low concentrations of WNT5A in the sample.
Target
WNT5A
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated WNT5A antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.044 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.16 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Wingless-type MMTV integration site family, member 5A
Alternate Names
Wingless-type MMTV integration site family, member 5A; hWNT5A; Protein Wnt-5a; WNT 5A protein precursor; WNT5A
Uniprot No.
P41221
N-Glycosylation Site
114, 120, 312, 326
Application Notes
Application
The quantitative human WNT5A (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of WNT5A, helping to understand its role in stem cells.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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