Recombinant Simian immunodeficiency virus (isolate PBj14/BCL-3) Envelope glycoprotein gp160 (ENV), Baculovirus

Recombinant Simian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) Envelope glycoprotein gp160 was expressed in Baculovirus with a His-tag or Tag free. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, and IP.

Determined by its binding ability in a functional ELISA.

Baculovirus

Simian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm)

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

ENV

Envelope glycoprotein gp160

The envelope glycoprotein gp160 precursor down-modulates cell surface CD4 antigen by interacting with it in the endoplasmic reticulum and blocking its transport to the cell surface. The gp120-gp41 heterodimer allows rapid transcytosis of the virus through CD4 negative cells such as simple epithelial monolayers of the intestinal, rectal and endocervical epithelial barriers. Both gp120 and gp41 specifically recognize glycosphingolipids galactosyl-ceramide (GalCer) or 3' sulfo-galactosyl-ceramide (GalS) present in the lipid rafts structures of epithelial cells. Binding to these alternative receptors allows the rapid transcytosis of the virus through the epithelial cells.

gp120; gp41; gp32