GlycoErase™ AMY1A Knockout HEK293T Cell Line

GlycoErase™ AMY1A knockout HEK293T cell line is a HEK293T model lacking AMY1A expression. AMY1A encodes a salivary isoform of alpha-amylase, a secreted enzyme responsible for hydrolyzing 1,4-alpha-glucosidic bonds in dietary starch and glycogen, initiating carbohydrate digestion. The knockout of AMY1A in the HEK293T background provides a tool to study amylase function, polysaccharide processing, and digestive enzyme regulation, and supports applications in carbohydrate metabolism analysis, enzyme function studies, and therapeutic enzyme research.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293T

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Penicillin/Streptomycin

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

AMY1A

Amylase Alpha 1A

AMY1

1p21.1

Amylases are secreted enzymes that break down 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, initiating the digestion of starch and glycogen. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing generates multiple transcript variants, encoding the same protein.