GlycoErase™ AMY1A Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF380)

Description	GlycoErase™ AMY1A knockout HEK293 cell line is a human embryonic kidney (HEK293) model lacking AMY1A expression. AMY1A encodes a salivary isoform of alpha-amylase, a secreted enzyme responsible for hydrolyzing 1,4-alpha-glucosidic bonds in dietary starch and glycogen, initiating carbohydrate digestion. The knockout of AMY1A in the HEK293 background provides a unique tool to study human digestive enzyme biology, regulation of polysaccharide breakdown, and extra-pancreatic expression of amylase, while supporting applications in metabolic research, enzyme replacement therapy modeling, and carbohydrate processing analysis.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AMY1A
Full Name	Amylase Alpha 1A
Alternative Name	AMY1
Location	1p21.1
Gene ID	276
Summary	This salivary gland amylase isoenzyme hydrolyzes 1,4-alpha-glucoside bonds in carbohydrates, initiating starch and glycogen digestion. It is part of a gene cluster expressed at high levels in salivary glands or pancreas. Alternative splicing generates identical protein products.