GlycoErase™ AMY1A Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF612)

Description	GlycoErase™ AMY1A knockout HepG2 cell line lacks AMY1A expression, a salivary isoform of α-amylase that hydrolyzes α-1,4-glucosidic bonds in starch and glycogen to initiate carbohydrate digestion. In HepG2 cells, this knockout supports research into hepatic amylase function, polysaccharide processing, and carbohydrate metabolism, with applications in metabolic disease, enzyme biology, and therapeutic enzyme evaluation.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	AMY1A
Full Name	Amylase Alpha 1A
Alternative Name	AMY1
Location	1p21.1
Gene ID	276
Summary	Amylases are secreted enzymes that hydrolyze 1,4-alpha-glucosidic bonds in starch and glycogen, initiating carbohydrate digestion. Multiple amylase genes are highly expressed in salivary glands or the pancreas. This gene encodes a salivary amylase isoenzyme, with alternative splicing generating multiple transcript variants of the same protein.