GlycoErase™ HEK293T GNE Knockout Cell Line (CAT#: GLJF-0425-JF435)

Description	We are pleased to present a GNE knockout HEK293T cell line for researchers studying sialic acid biosynthesis. This cell line lacks the GNE-encoded glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase, a bifunctional enzyme crucial for initiating and regulating the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. This model offers a unique opportunity to explore the consequences of disrupting sialic acid biosynthesis and its impact on cellular functions.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	GNE
Full Name	Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Alternative Name	IBM2, Uae1
Location	9p13.3
Gene ID	10020
Summary	Bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids; rate-limiting enzyme in the sialic acid pathway.