GlycoErase™ PDE3A Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF656)

Description	GlycoErase™ PDE3A knockout HepG2 cell line eliminates PDE3A, a cGMP-inhibited cyclic nucleotide phosphodiesterase that regulates intracellular cAMP and cGMP levels. PDE3A modulates vascular smooth muscle contraction and platelet aggregation, and is a target for heart failure therapies. This knockout enables studies of cyclic nucleotide regulation, cardiovascular signaling, and drug discovery in a human liver model.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PDE3A
Full Name	Phosphodiesterase 3A
Alternative Name	HTNB; CGI-PDE; CGI-PDE A; CGI-PDE-A
Location	12p12.2
Gene ID	5139
Summary	The encoded protein is a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase family, which hydrolyzes cAMP and cGMP to control signal duration and strength. It plays a role in platelet aggregation and vascular smooth muscle tone, making it a therapeutic target in heart failure. Multiple isoforms are produced via alternative splicing.