Description

GlycoFlux™ AAV1-ALG12 Viral Particle drives ALG12 to add the eighth mannose (α1,6) to dolichol-PP-Man₇GlcNAc₂, ensuring faithful N-glycan precursor assembly. Using AAV1 enables efficient transduction across cardiac and hepatic tissues as well as neural retina, auditory epithelium, pancreatic compartments, CNS, and smooth muscle for sustained output.

Product Type

Recombinant AAV1

Target

ALG12

Species

Human

Promotor

CMV, CAG, TRE, or other promoters

Tissue Tropism

Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver

Packaging System

AAV Triple-Plasmid Transfection System

Packaging Cell

HEK293T

Application

This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.

Shipping

Shipped on dry ice, with temperature maintained at ≤ -80°C.

Storage

Store at -80°C to preserve viral titer and stability.

Handling Notes

Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.

Titer Assay	qPCR/TCID50
Mycoplasma Test	No mycoplasma contamination was detected.
Sterility	Sterility testing verified the absence of microbial contamination.
Transduction Evaluation	Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.

Full Name	ALG12 alpha-1,6-mannosyltransferase
Alternative Name	ECM39; CDG1G
Location	22q13.33
Gene ID	79087
Summary	This gene encodes a protein that belongs to the glycosyltransferase 22 family. The protein plays a crucial role in protein glycosylation by catalyzing the addition of the eighth mannose residue to the dolichol pyrophosphate-linked oligosaccharide precursor (dolichol-PP-Man₇GlcNAc₂). This addition occurs via an alpha-1,6 linkage, and is essential for the proper assembly of the oligosaccharide chain required for N-linked glycoprotein synthesis.

GlycoFlux™ AAV1-Human ALG12 Viral Particle (CAT#: GLJF-1025-JF774)

Specifications

Quality Control

Target Information

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