GlycoFlux™ AAV1-Human ALG12 Viral Particle

GlycoFlux™ AAV1-ALG12 Viral Particle drives ALG12 to add the eighth mannose (α1,6) to dolichol-PP-Man₇GlcNAc₂, ensuring faithful N-glycan precursor assembly. Using AAV1 enables efficient transduction across cardiac and hepatic tissues as well as neural retina, auditory epithelium, pancreatic compartments, CNS, and smooth muscle for sustained output.

Recombinant AAV1

ALG12

Human

CMV, CAG, TRE, or other promoters

Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver

AAV Triple-Plasmid Transfection System

HEK293T

This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.

Shipped on dry ice, with temperature maintained at ≤ -80°C.

Store at -80°C to preserve viral titer and stability.

Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).

This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.

qPCR/TCID50

No mycoplasma contamination was detected.

Sterility testing verified the absence of microbial contamination.

Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.

All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.

ALG12 alpha-1,6-mannosyltransferase

ECM39; CDG1G

22q13.33

This gene encodes a protein that belongs to the glycosyltransferase 22 family. The protein plays a crucial role in protein glycosylation by catalyzing the addition of the eighth mannose residue to the dolichol pyrophosphate-linked oligosaccharide precursor (dolichol-PP-Man₇GlcNAc₂). This addition occurs via an alpha-1,6 linkage, and is essential for the proper assembly of the oligosaccharide chain required for N-linked glycoprotein synthesis.