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GlycoFlux™ MMLV-Human ALG12 Viral Particle

CAT#: GLJF-1025-JF260

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SPECIFIC INQUIRY
Antibiotic Resistance:
Fluorescent Reporters:
Titer:
Size:
Specifications Quality Control Target Information Related Products
Specifications
Description
GlycoFlux™ MMLV-ALG12 Viral Particle delivers ALG12, a GT-22 family enzyme that adds the eighth mannose (α1,6) to Dol-PP-Man7GlcNAc2 during N-glycan precursor synthesis. Intended for generating stable mammalian cell lines, it supports stepwise LLO reconstruction, antennary branching control, and functional testing of disease-associated alleles.
Product Type
Recombinant MMLV Retrovirus
Target
ALG12
Species
Human
Promotor
MMLV 5' LTR promoter
Packaging System
Retrovirus
Packaging Cell
HEK293T
Application
This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping
Shipped on dry ice with temperature held at or below -80°C.
Storage
Store at -80°C to maintain their titer and stability.
Handling Notes
Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer
These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Quality Control
Titer Assay
qPCR
Mycoplasma Test
Negative
Sterility
Sterility testing confirmed no microbial contamination.
Transduction Evaluation
On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation
Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Target Information
Full Name
ALG12 alpha-1,6-mannosyltransferase
Alternative Name
ECM39; CDG1G
Location
22q13.33
Gene ID
79087
Summary
Glycosyltransferase 22 family member that adds the eighth mannose to dolichol-PP-Man7GlcNAc2 via α1,6 linkage during N-glycan precursor synthesis.
For Research Use Only.
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