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GlycoFlux™ Lenti-Human PPP1CA Viral Particle (CAT#: GLJF-1025-JF209)

Datasheet

SPECIFIC INQUIRY
Antibiotic Resistance:
Fluorescent Reporters:
Titer:
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Description GlycoFlux™ Lenti-PPP1CA Viral Particle delivers PPP1CA, the PP1 catalytic α subunit that, with>200 regulatory partners, governs cell division, glycogen metabolism, muscle contraction, protein synthesis, and viral transcription. This platform enables creation of stable mammalian cell lines to dissect holoenzyme targeting, inhibitor sensitivity, and pathway cross-talk, using selection-based cloning or FACS.
Product Type Recombinant Lentivirus
Target PPP1CA
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name Protein Phosphatase 1 Catalytic Subunit Alpha
Alternative Name PP1A; PP-1A; PPP1A; PP1alpha
Location 11q13.2
Gene ID 5499
Summary This gene encodes one of three catalytic subunits of protein phosphatase 1 (PP1), the alpha subunit, which associates with over 200 regulatory proteins to form specific holoenzymes. PP1 regulates processes including cell division, glycogen metabolism, muscle contraction, protein synthesis, and viral transcription. Dysregulation is implicated in heart failure, diabetes, and cancer.
For Research Use Only.
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