Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ MMLV-AGL Viral Particle encodes AGL (glycogen debranching enzyme) with 4-α-glucotransferase and amylo-1,6-glucosidase activities critical for glycogen breakdown; this MMLV system builds stably transduced mammalian cells for glycogenolysis modeling and GSD variant complementation.

Product Type

Recombinant MMLV Retrovirus

Target

AGL

Species

Human

Promotor

MMLV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Insert Identity Confirmation

Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Target Information

Full Name

Amylo-Alpha-1, 6-Glucosidase, 4-Alpha-Glucanotransferase

Alternative Name

GDE

Location

1p21.2

Gene ID

178

Summary

Glycogen debranching enzyme is involved in glycogen breakdown, with 4-alpha-glucotransferase and amylo-1,6-glucosidase activities. Mutations in this gene lead to glycogen storage disease, with clinical variability potentially due to alternative splicing in different tissues.

GlycoFlux™ AAV1-Human AGL Viral Particle (CAT#: GLJF-1025-JF915)
GlycoFlux™ MSCV-Human AGL Viral Particle (CAT#: GLJF-1025-JF658)
GlycoFlux™ Lenti-Human AGL Viral Particle (CAT#: GLJF-1025-JF144)

GlycoFlux™ MMLV-Human AGL Viral Particle