Description

GlycoFlux™ MMLV-B3GLCT Viral Particle expresses B3GLCT, the β1,3-glucosyltransferase that modifies O-fucosylated TSRs; mutations cause Peters-plus syndrome. Purpose-built for establishing stable transductants in mammalian systems, it supports secretion/TSR function studies, complementation of loss-of-function lines, and targeted screening of TSR-dependent pathways.

Product Type

Recombinant MMLV Retrovirus

Target

B3GLCT

Species

Human

Promotor

MMLV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Full Name	Beta 3-glucosyltransferase
Alternative Name	B3Glc-T; B3GTL; B3GALTL
Location	13q12.3
Gene ID	145173
Summary	Beta-1,3-glucosyltransferase modifying O-fucosylated thrombospondin type-1 repeats; mutations lead to Peters-plus syndrome.

GlycoFlux™ MMLV-Human B3GLCT Viral Particle (CAT#: GLJF-1025-JF269)

Specifications

Quality Control

Target Information

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