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GlycoFlux™ AAV1-Human B3GLCT Viral Particle

CAT#: GLJF-1025-JF783

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Specifications
Description
GlycoFlux™ AAV1-B3GLCT Viral Particle drives robust B3GLCT expression to glucosylate O-fucosylated thrombospondin type-1 repeats, a pathway disrupted in Peters-plus syndrome, and this product achieves dependable delivery in retina and central nervous system tissues to support ocular and neurodevelopmental research.
Product Type
Recombinant AAV1
Target
B3GLCT
Species
Human
Promotor
CMV, CAG, TRE, or other promoters
Tissue Tropism
Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver
Packaging System
AAV Triple-Plasmid Transfection System
Packaging Cell
HEK293T
Application
This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.
Shipping
Shipped on dry ice, with temperature maintained at ≤ -80°C.
Storage
Store at -80°C to preserve viral titer and stability.
Handling Notes
Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer
This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.
Quality Control
Titer Assay
qPCR/TCID50
Mycoplasma Test
No mycoplasma contamination was detected.
Sterility
Sterility testing verified the absence of microbial contamination.
Transduction Evaluation
Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation
All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.
Target Information
Full Name
Beta 3-glucosyltransferase
Alternative Name
B3Glc-T; B3GTL; B3GALTL
Location
13q12.3
Gene ID
145173
Summary
Beta-1,3-glucosyltransferase that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats; defects cause Peters-plus syndrome.
For Research Use Only.
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