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GlycoFlux™ MSCV-Human PYGM Viral Particle (CAT#: GLJF-1025-JF762)

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Description GlycoFlux™ MSCV-PYGM Viral Particle installs muscle glycogen phosphorylase (PYGM) for skeletal-muscle glycogen usage studies and McArdle-associated variant testing; MSCV enables efficient, genome-integrating transduction for durable stable lines.
Product Type Recombinant MSCV Retrovirus
Target PYGM
Species Human
Promotor MSCV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.
Shipping Dispatched on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to preserve titer and stability.
Handling Notes Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.
Full Name Glycogen Phosphorylase, Muscle Associated
Alternative Name GSD5
Location 11q13.1
Gene ID 5837
Summary The muscle glycogen phosphorylase encoded by this gene is essential for skeletal muscle glycogen use. Similar forms are present in brain and liver. Mutations lead to McArdle disease, a glycogen storage disorder of muscle. Isoform diversity results from alternative splicing.
For Research Use Only.
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