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The GlycoSens™ Adenosine Deaminase (ADA) Assay Kit offers an advanced quantitative methodology for evaluating adenosine deaminase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Tissues, cellular lysates, other biological samples
Sample Volume
5 μL - 20 μL
Detection Principle
The system measures chromatic product formation derived from target adenosine deaminase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of ADA levels.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Adenosine Deaminase
Alternate Names
Adenosine Deaminase; ADA
Application Notes
Background
In the context of biological research, adenosine deaminase (ADA) has emerged as a molecule of high interest. Its enzymatic activity is finely tuned to environmental cues, reflecting the overall metabolic capacity. As a result, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.
Application
Designed for comprehensive functional analysis of adenosine deaminase (ADA) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.