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GlycoSens™ Calcium-Dependent Adenosine Triphosphatase (Ca2+-ATPase) Assay Kit

CAT#: GLJF-0926-HX132

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Specifications
Product Description
The GlycoSens™ Calcium-Dependent Adenosine Triphosphatase (Ca2+-ATPase) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of calcium-dependent adenosine triphosphatase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Oxidation and Antioxidant Series
Target
Ca2+-ATPase
Kit Components
Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution
Sample Types
Serum, plasma, tissue homogenates, cell lysates, biological fluids
Sample Volume
10 μL - 50 μL
Detection Principle
Target calcium-dependent adenosine triphosphatase (Ca2+-ATPase) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target Ca2+-ATPase.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Calcium-Dependent Adenosine Triphosphatase
Alternate Names
Calcium-Dependent Adenosine Triphosphatase; Ca2+-ATPase; Calcium-Dependent Adenosine Triphosphatase Standard; Calcium-Dependent Adenosine Triphosphatase Reagent
Application Notes
Background
In the context of biological research, calcium-dependent adenosine triphosphatase (Ca2+-ATPase) has emerged as a molecule of high interest. By driving active ion exchange, it supports electrical excitability and secondary active transport processes. Furthermore, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.
Application
Optimized for high-throughput profiling of calcium-dependent adenosine triphosphatase (Ca2+-ATPase) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.
Key Features & Advantages
High Specificity; Broad Sample Compatibility; Stringent Quality Control
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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