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The GlycoSens™ Calcium-Dependent Adenosine Triphosphatase (Ca2+-ATPase) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of calcium-dependent adenosine triphosphatase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Target calcium-dependent adenosine triphosphatase (Ca2+-ATPase) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target Ca2+-ATPase.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
In the context of biological research, calcium-dependent adenosine triphosphatase (Ca2+-ATPase) has emerged as a molecule of high interest. By driving active ion exchange, it supports electrical excitability and secondary active transport processes. Furthermore, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.
Application
Optimized for high-throughput profiling of calcium-dependent adenosine triphosphatase (Ca2+-ATPase) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.
Key Features & Advantages
High Specificity; Broad Sample Compatibility; Stringent Quality Control
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.