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The GlycoSens™ Glutamate Synthase (GOGAT) Assay Kit offers an advanced quantitative methodology for evaluating glutamate synthase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Other Series
Target
GOGAT
Kit Components
Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
The system measures chromatic product formation derived from target glutamate synthase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of GOGAT levels.
Detection Time
Within 45 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Glutamate Synthase
Alternate Names
Glutamate Synthase; GOGAT; Glutamate Synthetase
Application Notes
Background
Glutamate synthase (GOGAT) is an integral element required for robust cellular function and metabolism. It orchestrates the biosynthesis of complex biological molecules from simpler precursors. Notably, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.
Application
Optimized for high-throughput profiling of glutamate synthase (GOGAT) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.