GlycoSens™ Glutamate Synthase (GOGAT) Assay Kit, Micro-method

The GlycoSens™ Glutamate Synthase (GOGAT) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of glutamate synthase (GOGAT) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Other Series

GOGAT

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

5 μL - 20 μL

Quantification of glutamate synthase (GOGAT) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Glutamate Synthase

Glutamate Synthase; GOGAT; Glutamate Synthetase

The presence and activity of glutamate synthase (GOGAT) are closely linked to overall organismal health. By catalyzing the covalent linkage of its substrates, it supports intracellular synthesis requirements. In addition, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Designed for comprehensive functional analysis of glutamate synthase (GOGAT) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.