GlycoSens™ Glycogen Synthase (GCS) Assay Kit, Spectrophotometry

The GlycoSens™ Glycogen Synthase (GCS) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of glycogen synthase (GCS) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Glycogen Series

GCS

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

5 μL - 20 μL

Quantification of glycogen synthase (GCS) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Glycogen Synthase

Glycogen Synthase; GCS

Found widely across biological samples, glycogen synthase (GCS) is crucial for maintaining systemic homeostasis. By catalyzing the covalent linkage of its substrates, it supports intracellular synthesis requirements. Furthermore, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Essential for monitoring glycogen synthase (GCS) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.