GlycoSens™ Pepsin Assay Kit, Micro-method

The GlycoSens™ Pepsin Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target pepsin in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Protease Series

Pepsin

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

20 μL - 100 μL

The assay relies on the distinct spectroscopic optical characteristics of pepsin. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect pepsin content in the sample.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pepsin

Protease; Pepsin

Pepsin is an integral element required for robust cellular function and metabolism. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. Furthermore, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Tailored for accurate quantification of pepsin across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Linear Dynamic Range; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.