GlycoSens™ Pepsin Assay Kit, Spectrophotometry

The GlycoSens™ Pepsin Assay Kit offers an advanced quantitative methodology for evaluating pepsin concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Protease Series

Pepsin

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

50 μL

The system measures chromatic product formation derived from target pepsin activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of pepsin levels.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pepsin

Protease; Pepsin

Found widely across biological samples, pepsin is crucial for maintaining systemic homeostasis. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Moreover, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Ideal for investigating pepsin activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Superior Accuracy; Low Background Noise; Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.