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GlycoSens™ Sodium/Potassium-Transporting Adenosine Triphosphatase (Na+/K+-ATPase) Assay Kit, Spectrophotometry

CAT#: GLJF-0926-HX10

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The GlycoSens™ Sodium/Potassium-Transporting Adenosine Triphosphatase (Na+/K+-ATPase) Assay Kit delivers a robust spectrophotometric protocol for assessing Sodium/Potassium-Transporting Adenosine Triphosphatase (Na+/K+-ATPase) activity. The optimized reaction system minimizes background interference for streamlined workflow efficiency.
Product Type
Oxidative Phosphorylation Series
Target
Na+/K+-ATPase
Kit Components
Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution
Sample Types
Tissue homogenates, cell lysates, biological fluids
Sample Volume
10 μL - 50 μL
Detection Principle
Na+K+-ATPase breaks down ATP to produce ADP and inorganic phosphorus. The activity of ATPase is determined by measuring the amount of inorganic phosphorus.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Sodium/Potassium-Transporting Adenosine Triphosphatase
Alternate Names
Sodium/Potassium-Transporting Adenosine Triphosphatase; Na+/K+-ATPase; Sodium/Potassium-Transporting Adenosine Triphosphatase Standard
Application Notes
Background
The presence and activity of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) are closely linked to overall organismal health. Its activity is absolutely required for the proper functioning of muscle contraction and nerve impulse transmission. Investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.
Application
Ideal for investigating sodium/potassium-transporting adenosine triphosphatase (Na+K+-ATPase) activity and metabolic turnover during functional metabolomics, membrane gradient analysis, and metabolic homeostasis research.
Key Features & Advantages
Optimized Reaction Dynamics; Broad Sample Compatibility; Minimized Interference
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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