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Na+K+-ATPase breaks down ATP to produce ADP and inorganic phosphorus. The activity of ATPase is determined by measuring the amount of inorganic phosphorus.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Sodium/Potassium-Transporting Adenosine Triphosphatase; Na+/K+-ATPase; Sodium/Potassium-Transporting Adenosine Triphosphatase Standard
Application Notes
Background
Research highlights sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) as a significant participant in key metabolic and regulatory cascades. By driving active ion exchange, it supports electrical excitability and secondary active transport processes. Consequently, assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Optimized for high-throughput profiling of sodium/potassium-transporting adenosine triphosphatase (Na+K+-ATPase) in tissue bioenergetics, transmembrane gradient mechanics, and biochemical pathway modeling.
Key Features & Advantages
High Specificity; Broad Sample Compatibility; Ready-to-Use Reagents
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.