GlycoSens™ Starch Branching Enzyme (DBE) Assay Kit

The GlycoSens™ Starch Branching Enzyme (DBE) Assay Kit offers an advanced quantitative methodology for evaluating starch branching enzyme concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Carbohydrate Metabolism Series

DBE

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

5 μL - 20 μL

The system measures chromatic product formation derived from target starch branching enzyme activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of DBE levels.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Starch Branching Enzyme

Starch Branching Enzyme; DBE

Research highlights starch branching enzyme (DBE) as a significant participant in key metabolic and regulatory cascades. It serves as a fundamental energy source and a key structural component in biological tissues. Moreover, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Essential for monitoring starch branching enzyme (DBE) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.