GlycoSens™ Starch Debranching Enzyme (DBE) Assay Kit, Micro-method

The GlycoSens™ Starch Debranching Enzyme (DBE) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of starch debranching enzyme (DBE) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Starch Series

DBE

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

20 μL - 100 μL

Quantification of starch debranching enzyme (DBE) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Starch Debranching Enzyme

Starch Debranching Enzyme; DBE

Starch debranching enzyme (DBE) is recognized as a fundamental component in biological systems. As a primary metabolic intermediate, it fuels diverse biosynthetic and catabolic reactions. Accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Designed for comprehensive functional analysis of starch debranching enzyme (DBE) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.