GlycoSens™ Vitamin B1 (VB1) Assay Kit, Spectrophotometry

The GlycoSens™ Vitamin B1 (VB1) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of vitamin B1 (VB1) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Vitamin Series

VB1

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Cell lysates, tissue homogenates

20 μL - 100 μL

Quantification of vitamin B1 (VB1) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Vitamin B1

Vitamin B1; VB1

Known for its specific biological characteristics, vitamin B1 (VB1) provides deep insights into cellular dynamics. By participating as a cofactor, it bridges critical gaps in biosynthetic and regulatory pathways. In addition, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Optimized for high-throughput profiling of vitamin B1 (VB1) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.