GlycoSens™ Vitamin B1 (VB1) Assay Kit, Micro-method

The GlycoSens™ Vitamin B1 (VB1) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of vitamin B1. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Vitamin Series

VB1

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

Target vitamin B1 in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target VB1.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Vitamin B1

Vitamin B1; VB1

Found widely across biological samples, vitamin B1 (VB1) is crucial for maintaining systemic homeostasis. It prevents metabolic stalling by ensuring the structural stability and reactivity of vital enzymes. As a result, assessing this component offers a reliable parameter for agricultural research or biological studies.

Tailored for accurate quantification of vitamin B1 (VB1) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.