GlycoSens™ Vitamin B2 (VB2) Assay Kit

The GlycoSens™ Vitamin B2 (VB2) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of vitamin B2 (VB2) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Vitamin Series

VB2

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Serum, plasma, tissue homogenates, cell lysates, biological fluids

20 μL - 100 μL

Quantification of vitamin B2 (VB2) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

High-performance liquid chromatograph, centrifuge, chromatographic column, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Vitamin B2

Vitamin B2; VB2

Functioning at the core of various biological processes, vitamin B2 (VB2) is a key subject of biochemical analysis. By participating as a cofactor, it bridges critical gaps in biosynthetic and regulatory pathways. In addition, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.

Essential for monitoring vitamin B2 (VB2) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.